ABSTRACT
Objective:To establish an HPLC method for the simultaneous determination of rutin, salvianolic acid B and ginsen-oside Rb1 in Xinning tablets. Methods:The samples were separated on a CAPCELL PAK MG(250 mm × 4. 6 mm,5μm)column with gradient elution using acetonitrile(A) and 0. 1％ phosphoric acid(B) as the mobile phase at a flow rate of 1. 0 ml·min-1. The col-umn temperature was set at 35℃. The wavelengths were set on 354nm, 286nm and 203 nm (in a wavelength switching mode). Re-sults:The linear range of rutin was 0. 0273-1. 3600 mg·ml-1(r =1. 0000), that of salvianolic acid B was 0. 0244-1. 2200 mg· ml-1(r=1.0000),and that of ginsenoside Rb1 was 0.0186-0.9310 mg·ml-1(r=0.9999), and the average recovery (n=6) was 102.3％ (RSD=1.1％),98.7％ (RSD=0.8％) and 101.7％ (RSD=1.8％)(n=6), respectively. Conclusion: A rapid, simple, accurate HPLC method is successfully established for the simultaneous determination of 3 effective components in Xinning tab-lets, which is helpful to the quality control of Xinning tablets.
ABSTRACT
OBJECTIVE:To establish a method for the content determination of tanshinol,protocatechuic aldehyde,salvianolic acid B,paeoniflorin and ferulic acid in Xinning tablets. METHODS:Multi-target with multi-wavelength HPLC was performed on the column of Agilent Zorbax Eclipse XDB-C18 with the mobile phase of acetonitrile-methanol-0.5% H3PO4 aqueous solution(gradi-ent elution) at the flow rate of 1.0 ml/min. The column temperature was 30 ℃,volume was 10 μl and the detection wavelength was 280 nm for tanshinol,protocatechuic aldehyde and salvianolic acid B,230 nm for paeoniflorin and 320 nm for ferulic acid. RESULTS:The linear range was 66.25-1060.00 μg/ml for tanshinol(r=0.999 9),5.55-88.86 μg/ml for protocatechuic aldehyde (r=0.999 9),187.20-2 995.20 μg/ml for salvianolic acid B(r=0.999 7),23.71-379.39 μg/ml for paeoniflorin(r=0.999 9)and 0.20-3.12μg/ml for ferulic acid(r=0.999 7);the RSDs of precision,stability and repeatability tests were all less than 2%;the av-erage recoveries were respectively 98.85%(RSD=0.12%,n=6),97.95%(RSD=0.19%,n=6),99.18%(RSD=0.37%,n=6), 98.14%(RSD=0.25%,n=6) and 97.16%(RSD=1.36%,n=6). CONCLUSIONS:The method is simple and convenient with good separation effect,and can be used for the content determination of the 5 components in Xinning tablets.